GeneTex

  • Country / Location Selection

United States (US)

Ring1 antibody [8C12F4]

Anti-Ring1 antibody [8C12F4] used in Western Blot (WB). GTX60753
Anti-Ring1 antibody [8C12F4] used in Flow cytometry (FACS). GTX60753
Anti-Ring1 antibody [8C12F4] used in ELISA (ELISA). GTX60753
Anti-Ring1 antibody [8C12F4] used in Western Blot (WB). GTX60753
Anti-Ring1 antibody [8C12F4] used in IHC (Paraffin sections) (IHC-P). GTX60753
Anti-Ring1 antibody [8C12F4] used in IHC (Paraffin sections) (IHC-P). GTX60753

Cat. No. GTX60753

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8C12F4

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all Ring1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human Ring1 (AA: 79-263) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ring finger protein 1 , RING1A , RNF1

Cellular Localization

Nucleus,Nucleus speckle

Background

This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ring1 antibody [8C12F4] used in Western Blot (WB). GTX60753

GTX60753 WB Image

WB analysis of HEK293 (1) and Ring1 (AA: 79-263)-hIgGFc transfected HEK293 (2) cell lysate using GTX60753 Ring1 antibody [8C12F4].

Anti-Ring1 antibody [8C12F4] used in Flow cytometry (FACS). GTX60753

GTX60753 FACS Image

FACS analysis of HeLa cells using GTX60753 Ring1 antibody [8C12F4].
Green : Ring1
Red : negative control

Anti-Ring1 antibody [8C12F4] used in ELISA (ELISA). GTX60753

GTX60753 ELISA Image

ELISA analysis of antigen using GTX60753 Ring1 antibody [8C12F4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Ring1 antibody [8C12F4] used in Western Blot (WB). GTX60753

GTX60753 WB Image

WB analysis of MOLT-4 (1), LNCaP (2), HeLa (3), HEK-293 (4) and Jurkat (5) cell lysate using GTX60753 Ring1 antibody [8C12F4].

Anti-Ring1 antibody [8C12F4] used in IHC (Paraffin sections) (IHC-P). GTX60753

GTX60753 IHC-P Image

IHC-P analysis of rectum cancer tissue using GTX60753 Ring1 antibody [8C12F4].

Anti-Ring1 antibody [8C12F4] used in IHC (Paraffin sections) (IHC-P). GTX60753

GTX60753 IHC-P Image

IHC-P analysis of cervical cancer tissue using GTX60753 Ring1 antibody [8C12F4].

REFERENCE

There are currently no references for Ring1 antibody [8C12F4] (GTX60753). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Ring1 antibody [8C12F4] (GTX60753). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409