APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 2.5μg/ml |
|
Note :
IHC-P
Human Lung shows nuclear staining in select cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88481-PEP).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-DTNADKQLSFEEF , from the internal region of the protein sequence according to NP_002956.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
S100 calcium binding protein A9 , 60B8AG , CAGB , CFAG , CGLB , L1AG , LIAG , MAC387 , MIF , MRP14 , NIF , P14
Cellular Localization
Secreted , Cytoplasm , Cytoplasm, cytoskeleton
Background
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. This antimicrobial protein exhibits antifungal and antibacterial activity. [provided by RefSeq, Nov 2014]
Database
Research Area
DATA IMAGES
|
GTX88481 WB Image
WB analysis of human peripheral mononucleocytes lysate using GTX88481 S100A9 antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
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|
GTX88481 IHC-P Image
IHC-P analysis of human liver using GTX88481 S100A9 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
|
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GTX88481 IHC-P Image
IHC-P analysis of human lung using GTX88481 S100A9 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
|
REFERENCE
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REVIEW
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