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SNF8 antibody

Anti-SNF8 antibody used in IHC (Whole mount) (IHC-Wm). GTX119948
Anti-SNF8 antibody used in Western Blot (WB). GTX119948
Anti-SNF8 antibody used in IHC (Paraffin sections) (IHC-P). GTX119948

Cat. No. GTX119948

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all SNF8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SNF8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618073

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SNF8 subunit of ESCRT-II , Dot3 , EAP30 , VPS22

Cellular Localization

Cytoplasm (Probable) , Nucleus

Background

SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SNF8 antibody used in IHC (Whole mount) (IHC-Wm). GTX119948

GTX119948 IHC-Wm Image

SNF8 antibody detects Snf8 protein on blood cells by immunohistochemical analysis Sample: Whole-mount zebrafish embryo SNF8 antibody (GTX119948) dilution: 1:200.

Anti-SNF8 antibody used in Western Blot (WB). GTX119948

GTX119948 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
12% SDS PAGE
GTX119948 diluted at 1:1000

Anti-SNF8 antibody used in IHC (Paraffin sections) (IHC-P). GTX119948

GTX119948 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SNF8(GTX119948) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169