APPLICATION
Application Note
Dot Blot : 1.0 μg/ml
WB : 0.5 to 2.0 μg/ml
Optimal dilutions / concentrations should be determined by the end user.
Calculated MW
Product Note
This antibody reacts predominantly with phosphorylated SOX 9. The antibodies were evaluated for specificity with a dot blot assay using synthetically prepared SOX 9 peptides. It only recognizes the phosphorylated serine 181 of human SOX 9,not other phosphorylated sites or non-phosphorylated SOX 9.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide (QPRRRKpSVKNG)corresponding to human / mouse / bovine SOX 9 at thephosphorylation site of Serine 181.
Purification
Immunogen affinity purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SRY-box 9 , CMD1 , CMPD1 , SRA1 , SRXX2 , SRXY10
Cellular Localization
Nucleus
Background
The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX23696 Dot Image
Dot blot analysis of the SOX-9 peptide (pSer181) and SOX-9 peptide probed with the anti-SOX-9 (pSer181) (GTX23696) and anti-SOX-9 (GTX23697). The staining shows that the anti-SOX-9 (pSer181) recognized the SOX-9 peptide (pSer181) only; whole the anti-SOX-9 could bind both the SOX-9 peptide (pSer181) and SOX-9 peptide.
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REFERENCE
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REVIEW
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