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STING antibody

KO/KD Orthogonal
Anti-STING antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134373
Anti-STING antibody used in IHC (Paraffin sections) (IHC-P). GTX134373
Anti-STING antibody used in Western Blot (WB). GTX134373

Cat. No. GTX134373

3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MPYS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MPYS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887262

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transmembrane protein 173 , ERIS , MITA , MPYS , NET23 , SAVI , STING , STING-beta , hMITA , hSTING

Cellular Localization

Endoplasmic reticulum membrane,Mitochondrion outer membrane,Cell membrane,Cytoplasm

Background

This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Database

Research Area

DATA IMAGES

Anti-STING antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134373

GTX134373 ICC/IF Image

STING antibody detects STING protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: STING stained by STING antibody (GTX134373) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-STING antibody used in IHC (Paraffin sections) (IHC-P). GTX134373

GTX134373 IHC-P Image

STING antibody detects STING protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
STING stained by STING antibody (GTX134373) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-STING antibody used in Western Blot (WB). GTX134373

GTX134373 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with STING antibody (GTX134373) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

There are currently no references for STING antibody (GTX134373). Be the first to share your publications with this product.
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REVIEW

STING antibody Cat. No. GTX134373

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-STING antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134373
Date : Anonymous submitted on 28-Jun-2024
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : PMA-treated THP-1 WT and STING1 KO cells
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description :
Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown.
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-STING antibody used in Immunoprecipitation (IP). GTX134373
Date : Anonymous submitted on 28-Jun-2024
Score :
Application Tested : IP
Sample Species : Hu
Sample : THP-1 cells
Lane Description :
SM=4% starting material
UB=4% unbound fraction
Figure Note :
Antibody-beads conjugates were prepared by adding 2 μg of antibody to 500 μl of IP Lysis Buffer in a microcentrifuge tube, together with with 30μl of Dynabeads protein A- (for rabbit antibodies) or protein G- (for mouse antibodies) beads. Tubes were rocked for ~1 hr at 4ºC followed by two washes to remove unbound antibodies.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-STING antibody used in Western Blot (WB). GTX134373
Date : Anonymous submitted on 28-Jun-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : THP-1 WT and STING KO cells
Amount used : 40μg
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169