STK3 antibody [4G10]

Name: STK3 antibody [4G10]
Brand: GeneTex
SKU: GTX83546
Price: 519.00 USD

Anti-STK3 antibody [4G10] used in IHC (Paraffin sections) (IHC-P). GTX83546

Anti-STK3 antibody [4G10] used in Western Blot (WB). GTX83546

Cat. No. GTX83546

Host	Mouse
Clonality	Monoclonal
Clone Name	4G10
Isotype	IgG2b
Application	WB, IHC-P
Reactivity	Human, Mouse, Dog, Monkey
Package	100 μl ($519)

See all STK3 products

Datasheet
Document

View product citations for antibody GTX83546 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
IHC-P	1:50

Not tested in other applications.

Calculated MW

56 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human STK3 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serine/threonine kinase 3 , KRS1 , MST2

Cellular Localization

Cytoplasm,Nucleus

Background

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1.

Database

Gene ID: 6788 STK3
UniProt: Q13188 STK3

Research Area

DATA IMAGES

GTX83546 IHC-P Image

IHC-P analysis of ovary tissue using GTX83546 STK3 antibody [4G10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83546 IHC-P Image

IHC-P analysis of endometrium tissue using GTX83546 STK3 antibody [4G10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83546 WB Image

WB analysis of various samples using GTX83546 STK3 antibody [4G10].
Loading : 10 ug per lane
Dilution : 1:200

GTX83546 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83546 STK3 antibody [4G10].
Loading : 10 ug per lane
Dilution : 1:200

GTX83546 IHC-P Image

IHC-P analysis of lung tissue using GTX83546 STK3 antibody [4G10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83546 IHC-P Image

IHC-P analysis of kidney carcinoma tissue using GTX83546 STK3 antibody [4G10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83546 IHC-P Image

IHC-P analysis of kidney tissue using GTX83546 STK3 antibody [4G10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83546 WB Image

WB analysis of HEK293T cells transfected with STK3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83546 STK3 antibody [4G10].
Loading : 5 ug per lane

GTX83546 WB Image

WB analysis of various cell lines using GTX83546 STK3 antibody [4G10].
Loading : 35 ug per lane

GTX83546 IHC-P Image

IHC-P analysis of liver tissue using GTX83546 STK3 antibody [4G10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83546 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX83546 STK3 antibody [4G10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83546 IHC-P Image

IHC-P analysis of colon tissue using GTX83546 STK3 antibody [4G10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83546 IHC-P Image

IHC-P analysis of pancreas tissue using GTX83546 STK3 antibody [4G10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

STK3 antibody [4G10]

Cat. No. GTX83546

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX83546 IHC-P Image

GTX83546 IHC-P Image

GTX83546 WB Image

GTX83546 WB Image

GTX83546 IHC-P Image

GTX83546 IHC-P Image

GTX83546 IHC-P Image

GTX83546 WB Image

GTX83546 WB Image

GTX83546 IHC-P Image

GTX83546 IHC-P Image

GTX83546 IHC-P Image

GTX83546 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.