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TIM-3 antibody [1827CT151.74.68]

Anti-TIM-3 antibody [1827CT151.74.68] used in Western Blot (WB). GTX04870
Anti-TIM-3 antibody [1827CT151.74.68] used in Flow cytometry (FACS). GTX04870

Cat. No. GTX04870

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1827CT151.74.68

Isotype

 IgG2b

Application

 WB, FACS

Reactivity

 Human
Package
200 μl ($399)
See all TIM-3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:4000
FACS 1:25
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein of human HAVCR2.

Purification

Purified by Protein G followed by dialysis against PBS

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD366,HAVCR2,HAVcr2,KIM3,TIM3,TIMD3,Tim3,hepatitis A virus cellular receptor 2,TIM-3

Cellular Localization

Membrane,Cell junction

Background

The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-TIM-3 antibody [1827CT151.74.68] used in Western Blot (WB). GTX04870

GTX04870 WB Image

WB analysis of various samples using GTX04870 TIM-3 antibody [1827CT151.74.68].
Lane 1 : HepG2 whole cell lysate
Lane 2 : Daudi whole cell lysate
Lane 3 : Human kidney lysate Lysate
Loading : 20 μg
Dilution : 1:4000

Anti-TIM-3 antibody [1827CT151.74.68] used in Flow cytometry (FACS). GTX04870

GTX04870 FACS Image

FACS analysis of 2% PFA fixed U2OS cells using GTX04870 TIM-3 antibody [1827CT151.74.68].
Green line : Primary antibody
Blue line : Isotype control
Dilution : 1:25

REFERENCE

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REVIEW

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Package List Price ($)
$ 399