APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 2 μg/mL |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat Cell Slide(GTX25533) , Jurkat Cell Lysate(GTX27899)
Product Note
At least two isoforms of TLR9 are known to exist; this antibody will detect both isoforms. TLR9 antibody is predicted to not cross-react with other TLR proteins.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
TLR9 antibody was raised against a peptide corresponding to 15 amino acids near the center of human TLR9.The immunogen is located within amino acids 840 - 890 of TLR9.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
toll like receptor 9 , CD289
Cellular Localization
Endoplasmic reticulum membrane,Endosome,Lysosome
Background
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31295 WB Image
WB analysis of Jurkat cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31295 TLR9 antibody.
Working concentration : 1 μg/ml
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GTX31295 ICC/IF Image
ICC/IF analysis of Jurkat cells using GTX31295 TLR9 antibody.
Working concentration : 2 μg/ml
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GTX31295 IHC-P Image
IHC-P analysis of mouse spleen tissue using GTX31295 TLR9 antibody.
Working concentration : 10 μg/ml
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REFERENCE
There are currently no references for TLR9 antibody (GTX31295). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TLR9 antibody (GTX31295). Be the first to share your experience with this product.
