APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.1-0.3μg/ml |
10μg/ml |
Note :
IHC-P
Human Kidney, shows diffuse cytopasmic staining in epithelial cells of renal tubules.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX19045-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_005700.2 and NP_710142.1).
Predict Reactivity
Mouse, Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence DRKVAREFRHKVD-C, from the N Terminus of the protein sequence according to NP_005700.2; NP_710142.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
USH1 protein network component harmonin , AIE-75 , DFNB18 , DFNB18A , NY-CO-37 , NY-CO-38 , PDZ-45 , PDZ-73 , PDZ-73/NY-CO-38 , PDZ73 , PDZD7C , ush1cpst
Cellular Localization
Cytoplasm, cytosol , Cytoplasm, cytoskeleton
Background
This gene encodes a scaffold protein that functions in the assembly of Usher protein complexes. The protein contains PDZ domains, a coiled-coil region with a bipartite nuclear localization signal and a PEST degradation sequence. Defects in this gene are the cause of Usher syndrome type 1C and non-syndromic sensorineural deafness autosomal recessive type 18. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Database
Research Area
DATA IMAGES
|
GTX19045 IHC-P Image
IHC-P analysis of human kidney using GTX19045 USH1C antibody. Antigen retrieval : citrate buffer pH 6 Dilution : 10μg/ml
|
|
GTX19045 WB Image
WB analysis of HEK293 lysate using GTX19045 USH1C antibody. Dilution : 0.1μg/ml Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for USH1C antibody (GTX19045). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for USH1C antibody (GTX19045). Be the first to share your experience with this product.