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Wnt5b antibody, Internal

Anti-Wnt5b antibody, Internal used in Western Blot (WB). GTX81146
Anti-Wnt5b antibody, Internal used in Flow cytometry (FACS). GTX81146
Anti-Wnt5b antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81146

Cat. No. GTX81146

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse
Package
400 μl ($399)
See all Wnt5b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 153-182 amino acids from the Central region of human WNT5B.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 5B

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Wnt5b antibody, Internal used in Western Blot (WB). GTX81146

GTX81146 WB Image

WB analysis of mouse bladder tissue lysate (35ug/lane) using GTX81146 WNT5B antibody, Internal.

Anti-Wnt5b antibody, Internal used in Flow cytometry (FACS). GTX81146

GTX81146 FACS Image

FACS analysis of WiDr cells using GTX81146 WNT5B antibody, Internal.
Top histogram : negative control
Bottom histogram : WiDr cells

Anti-Wnt5b antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81146

GTX81146 IHC-P Image

IHC-P analysis of human skeletal muscle using GTX81146 WNT5B antibody, Internal.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399