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YAP1 antibody [GT256]

KO/KD
Anti-YAP1 antibody [GT256] used in Western Blot (WB). GTX633541
Anti-YAP1 antibody [GT256] used in IHC (Paraffin sections) (IHC-P). GTX633541
Anti-YAP1 antibody [GT256] used in IHC (Paraffin sections) (IHC-P). GTX633541
Anti-YAP1 antibody [GT256] used in Western Blot (WB). GTX633541

Cat. No. GTX633541

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT256

Isotype

 IgG2a

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all YAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HepG2 , HeLa

Predict Reactivity

Rat, Zebrafish, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human YAP1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888344

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Yes associated protein 1 , COB1 , YAP , YAP2 , YAP65 , YKI

Background

This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-YAP1 antibody [GT256] used in Western Blot (WB). GTX633541

GTX633541 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody [GT256] (GTX633541) diluted at 1:500.

Anti-YAP1 antibody [GT256] used in IHC (Paraffin sections) (IHC-P). GTX633541

GTX633541 IHC-P Image

YAP1 antibody [GT256] detects YAP1 protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
YAP1 antibody [GT256] (GTX633541) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-YAP1 antibody [GT256] used in IHC (Paraffin sections) (IHC-P). GTX633541

GTX633541 IHC-P Image

YAP1 antibody [GT256] detects YAP1 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
YAP1 antibody [GT256] (GTX633541) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-YAP1 antibody [GT256] used in Western Blot (WB). GTX633541

GTX633541 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody [GT256] (GTX633541) diluted at 1:1000.

REFERENCE

Application Reference

J Mohamed et al. BPJ 2024; Clinical, Molecular Subgroups and Survival Rates Finding of Childhood Medulloblastoma: A Ten Years Moroccan Experience in Pediatric Hematology and Oncology Center.
Application : IHC-P
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations