Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:2000 |
1:100-1:500 |
1:50-1:200 |
Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Zebrafish, Rabbit, Sheep, Chicken, Pig, Monkey, Horse, Xenopus(>80% identity)
Form
Liquid
Buffer
PBS, 150 mM NaCl, 50% glycerol
Preservative
0.02% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human YAP1(Accession P46937), corresponding to amino acid residues around phosphorylated Ser127.
Purification
From serum
Purity
Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
COB1,YAP,YAP1,YAP2,YAP65,YKI,Yes associated protein 1
Cellular Localization
Cytoplasm,Nucleus
Background
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]
Database
Research Area