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Zika virus Envelope protein antibody

Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133326
Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133326
Anti-Zika virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133326
Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133326
Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133326
Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133326
Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133326
Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133326
Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133326
Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133326

Cat. No. GTX133326

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

Zika virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX634155 / GTX634157 / GTX133326, Detection : GTX133326 / GTX634157 / GTX634155

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Zika virus Envelope-transfected 293T , Zika virus Envelope-infected C6/36

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Zika virus Envelope protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886931

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Envelope , ZIKV Envelope

Research Area

DATA IMAGES

Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133326

GTX133326 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX133326 as capture antibody at concentration of 5 μg/mL and GTX634155 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133326

GTX133326 ELISA Image

Sandwich ELISA detection of Zika virus-infected lysate using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133326 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133326

GTX133326 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus Envelope protein antibody (GTX133326).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus Envelope protein antibody (GTX133326) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133326

GTX133326 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634157 as capture antibody at concentration of 5 μg/mL and GTX133326 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133326

GTX133326 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX133326 as capture antibody at concentration of 5 μg/mL and GTX634157 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133326

GTX133326 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133326 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133326

GTX133326 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133326) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133326

GTX133326 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133326) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133326

GTX133326 WB Image

The data was published in the 2019 in Sci Rep. PMID: 31534148

Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133326

GTX133326 WB Image

The data was published in the 2019 in Sci Rep. PMID: 31534148

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169