GeneTex

  • Country / Location Selection

United States (US)

alpha 1 Antitrypsin antibody (HRP)

Anti-alpha 1 Antitrypsin antibody (HRP) used in ELISA (ELISA). GTX27635

Cat. No. GTX27635

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, IHC

Reactivity

 Human
Package
1 mg ($459)
See all alpha 1 Antitrypsin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
ELISA 1:4000-1:20000
IHC 1:500-1:2500
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Gentamicin Sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Alpha 1-Anti-Trypsin collected from human plasma

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_372991

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serpin family A member 1 , A1A , A1AT , AAT , PI , PI1 , PRO2275 , alpha1AT , nNIF

Cellular Localization

Secreted,Endoplasmic reticulum

Background

Alpha 1 Antitrypsin is a plasma protein synthesised in the liver. It consists of a single polypeptide chain and has a molecular weight of 52 kDa. A serine protease inhibitor, its primary function is the protection of lung tissue from the action of neutrophil elastase. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT (SPAAT) is a reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). The average level of alpha 1 Antitrypsin in plasma is 1.3 g/l.

Database

Research Area

DATA IMAGES

Anti-alpha 1 Antitrypsin antibody (HRP) used in ELISA (ELISA). GTX27635

GTX27635 ELISA Image

ELISA analysis of human alpha 1 antitrypsin protein using serially diluted GTX27635 alpha 1 Antitrypsin antibody (HRP).
Coating : 1 μg

REFERENCE

Application Reference

Lin G et al. J Sex Med 2010; 7 (5) : 1787-97 Pentoxifylline attenuates transforming growth factor-beta1-stimulated elastogenesis in human tunica albuginea-derived fibroblasts part 2: Interference in a TGF-beta1/Smad-dependent mechanism and downregulation of AAT1.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for alpha 1 Antitrypsin antibody (HRP) (GTX27635). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 459