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alpha 1 Antitrypsin antibody

Anti-alpha 1 Antitrypsin antibody used in Western Blot (WB). GTX27633
Anti-alpha 1 Antitrypsin antibody used in Western Blot (WB). GTX27633
Anti-alpha 1 Antitrypsin antibody used in Western Blot (WB). GTX27633
Anti-alpha 1 Antitrypsin antibody used in Western Blot (WB). GTX27633
Anti-alpha 1 Antitrypsin antibody used in Western Blot (WB). GTX27633

Cat. No. GTX27633

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human
Package
1 mg ($399)
See all alpha 1 Antitrypsin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000-1:10000
ELISA 1:20000-1:100000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Alpha 1-Anti-Trypsin collected from human plasma

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serpin family A member 1 , A1A , A1AT , AAT , PI , PI1 , PRO2275 , alpha1AT , nNIF

Cellular Localization

Secreted,Endoplasmic reticulum

Background

alpha 1 Antitrypsin is a plasma protein synthesised in the liver. It consists of a single polypeptide chain and has a molecular weight of 52 kDa. A serine protease inhibitor, its primary function is the protection of lung tissue from the action of neutrophil elastase. The average level of alpha 1 Antitrypsin in plasma is 1.3 g/l.

Database

Research Area

DATA IMAGES

Anti-alpha 1 Antitrypsin antibody used in Western Blot (WB). GTX27633

GTX27633 WB Image

Western Blot of Goat anti Alpha-1anti-Trypsin antibody. Lane 1: Alpha-1anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 µg per lane. Primary antibody: Alpha-1anti-Trypsin antibody (GTX27633) at 1:3000 for overnight at 4°C. Secondary antibody: Flourescent dye 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: overnight at 4°C. Predicted/Observed size: 55kDa for a-1 Anti-Trypsin. Other band(s): none

Anti-alpha 1 Antitrypsin antibody used in Western Blot (WB). GTX27633

GTX27633 WB Image

WB analysis of human alpha 1 antitrypsin protein using GTX27633 alpha 1 Antitrypsin antibody.
Loading : 1 μg
Dilution : 1:3000

Anti-alpha 1 Antitrypsin antibody used in Western Blot (WB). GTX27633

GTX27633 WB Image

WB analysis of human serum using GTX27633 alpha 1 Antitrypsin antibody.
Red : Primary antibody
Green : Transferrin
Blue : Human IgG
Loading : 35 μg
Dilution : 1:1000

Anti-alpha 1 Antitrypsin antibody used in Western Blot (WB). GTX27633

GTX27633 WB Image

WB analysis of human serum with GST using GTX27633 alpha 1 Antitrypsin antibody.
Red : Primary antibody
Green : Transferrin
Blue : GST
Loading : 35 μg
Dilution : 1:1000

Anti-alpha 1 Antitrypsin antibody used in Western Blot (WB). GTX27633

GTX27633 WB Image

WB analysis of albumin depleted human serum using GTX27633 alpha 1 Antitrypsin antibody.
Loading : 10 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399