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beta 2 Microglobulin antibody (HRP)

Anti-beta 2 Microglobulin antibody (HRP) used in Western Blot (WB). GTX40576
Anti-beta 2 Microglobulin antibody (HRP) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX40576
Anti-beta 2 Microglobulin antibody (HRP) used in IHC (Paraffin sections) (IHC-P). GTX40576
Anti-beta 2 Microglobulin antibody (HRP) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX40576
Anti-beta 2 Microglobulin antibody (HRP) used in Western Blot (WB). GTX40576
Anti-beta 2 Microglobulin antibody (HRP) used in IHC (Paraffin sections) (IHC-P). GTX40576

Cat. No. GTX40576

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
250 μg ($399)
See all beta 2 Microglobulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:200
IHC-P 1:200-1:1000
ELISA 1:2000-1:10000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Gentamicin Sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Beta-2-Microglobulin protein isolated from human urine.

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

beta-2-microglobulin , IMD43

Cellular Localization

Secreted

Background

Beta 2 Microglobulin non covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Database

Research Area

DATA IMAGES

Anti-beta 2 Microglobulin antibody (HRP) used in Western Blot (WB). GTX40576

GTX40576 WB Image

WB analysis using beta 2 Microglobulin antibody (HRP). Load: 50 ng Beta-2-Microglobulin. Predicted/Observed size: 12 kDa.

Anti-beta 2 Microglobulin antibody (HRP) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX40576

GTX40576 ICC/IF Image

ICC/IF analysis of 2% PFA fixed HEK293 cells using GTX40576 beta 2 Microglobulin antibody (HRP).
Dilution : 1:200
Secondary antibody : donkey anti-rabbit secondary antibody conjugated with Alexa Fluor 488.

Anti-beta 2 Microglobulin antibody (HRP) used in IHC (Paraffin sections) (IHC-P). GTX40576

GTX40576 IHC-P Image

IHC-P analysis of human skin tissue using Isotype control (left) and beta 2 Microglobulin antibody (HRP) at a concentration of 1 ug/ml (right).

Anti-beta 2 Microglobulin antibody (HRP) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX40576

GTX40576 ICC/IF Image

ICC/IF analysis of 2%PFA-fixed 293T cells using GTX40576 beta 2 Microglobulin antibody (HRP).
Dilution : 1:200

Anti-beta 2 Microglobulin antibody (HRP) used in Western Blot (WB). GTX40576

GTX40576 WB Image

WB analysis of human beta 2 microglobulin protein using GTX40576 beta 2 Microglobulin antibody (HRP).
Loading : 50 ng
Dilution : 1:1000

Anti-beta 2 Microglobulin antibody (HRP) used in IHC (Paraffin sections) (IHC-P). GTX40576

GTX40576 IHC-P Image

IHC-P analysis of human skin tissue using GTX40576 beta 2 Microglobulin antibody (HRP).
Left panel : Isotype control
Right panel : Primary antibody
Dilution : 1 μg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 399