APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa (25 μg/ml Anisomycin treatment for 30 min) , 293T , A431 , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2
Predict Reactivity
Zebrafish, Rabbit, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.95 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser73 of human c-Jun. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2887128
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Jun proto-oncogene, AP-1 transcription factor subunit , AP-1 , AP1 , c-Jun , p39
Cellular Localization
Nucleus
Background
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX133873 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133873 IHC-P Image
c-Jun (phospho Ser73) antibody detects c-Jun (phospho Ser73) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.c-Jun (phospho Ser73) stained by c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133873 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133873 IHC-P Image
c-Jun (phospho Ser73) antibody detects c-Jun (phospho Ser73) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse lung.c-Jun (phospho Ser73) stained by c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133873 ICC/IF Image
c-Jun (phospho Ser73) antibody detects c-Jun (phospho Ser73) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: c-Jun (phospho Ser73) stained by c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10μm.
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GTX133873 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500 and competitor's antibody (CST#3270) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX133873 IHC-P Image
c-Jun (phospho Ser73) antibody detects c-Jun (phospho Ser73) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.c-Jun (phospho Ser73) stained by c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133873 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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