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c-Myc (phospho Ser62) antibody [33A12E10]

KO/KD
Anti-c-Myc (phospho Ser62) antibody [33A12E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00684
Anti-c-Myc (phospho Ser62) antibody [33A12E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00684
Anti-c-Myc (phospho Ser62) antibody [33A12E10] used in Western Blot (WB). GTX00684

Cat. No. GTX00684

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 33A12E10

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, IP, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all c-Myc products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB ~1 μg/ml
ICC/IF 0.5-1 μg/ml
IHC-P 5 μg/ml
FACS using 1 μg for 10⁶ cells.
IP Assay dependent
ELISA Assay dependent

Note :

IHC-P
Recommed using a heat mediated antigen retrieval with citrate buffer (pH 6.0).
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide containing phospho-Ser62 of cMyc

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MYC proto-oncogene, bHLH transcription factor , MRTL , MYCC , bHLHe39 , c-Myc

Cellular Localization

nucleoplasm,Nucleolus

Background

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-c-Myc (phospho Ser62) antibody [33A12E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00684

GTX00684 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00684 c-Myc (phospho Ser62) antibody [33A12E10].
Dilution : 1:2000 in 1% BSA in PBS at 4 degree C, overnight
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS for 10 min

Anti-c-Myc (phospho Ser62) antibody [33A12E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00684

GTX00684 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00684 c-Myc (phospho Ser62) antibody [33A12E10] (Green, Left) or proximity Ligation Analysis with anti-c-Myc pS62 and CIP2A antibodies, association of c-Myc pS62 with CIP2A (Right, red) in nuclei (DAPI, blue).

Anti-c-Myc (phospho Ser62) antibody [33A12E10] used in Western Blot (WB). GTX00684

GTX00684 WB Image

KO/KD

WB analysis of various samples using GTX00684 c-Myc (phospho Ser62) antibody [33A12E10].
Scrambled siRNA : AGS cells transfected with scrambled siRNA
Negative control siRNA : AGS cells transfected with negative control siRNA
Myc1 : AGS cells transfected with c-Myc siRNA

REFERENCE

Application Reference

Lee CC et al. Life Sci 2021; 277 : 119438 A regulatory loop among CD276, miR-29c-3p, and Myc exists in cancer cells against natural killer cell cytotoxicity.
Application : WB

Clone Reference

Myant K et al. Cell Rep. 2015; 12 (6):1019-31 Serine 62-Phosphorylated MYC Associates with Nuclear Lamins and Its Regulation by CIP2A Is Essential for Regenerative Proliferation.
Application : WB, ICC/IF
Reactivity : Human, Mouse
Mathiasen DP et al. Oncogene. 2012; 31 (3):390-401 Identification of a c-Jun N-terminal kinase-2-dependent signal amplification cascade that regulates c-Myc levels in ras transformation.
Application : WB
Reactivity : Mouse
Tibbitts DC et al. Blood. 2012; 119 (22) Studying c-Myc serine 62 phosphorylation in leukemia cells: concern over antibody cross-reactivity.
Application : WB, IP
Reactivity : Human
Tallents RH et al. Oral Health. 1988; 78 (2):29-33 Provisional restorations. Part Two.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399