APPLICATION
Application Note
For IHC-Fr: Use at a concentration of 10 μg/ml. For IHC-P: Use at a concentration of 10 μg/ml. For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 5 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Positive Control
SW480, Y1 cells, or normal skin tissue
Product Note
This antibody reacts with cK Ras and vK Ras, and cross-reacts with cH Ras or cN Ras p21s in immunoblotting. For this reason we recommend a sample concentration step prior to immunoblotting; concentrate to 5 μg/ml with 1% gelatin as a stabilizer. For immunoprecipitation, use 5 μg per sample with 45 μl protein A agarose.
PROPERTIES
Form
Liquid
Buffer
50mM Sodium Phosphate, 0.2% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Rat
Immunogen
Recombinant fragment, corresponding to C terminal amino acids 54-189 of Rat cK RAS.
Purification
Purified IgG
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
KRAS proto-oncogene, GTPase , C-K-RAS , CFC2 , K-RAS2A , K-RAS2B , K-RAS4A , K-RAS4B , K-Ras , KI-RAS , KRAS1 , KRAS2 , NS , NS3 , RALD , RASK2 , c-Ki-ras2
Cellular Localization
Cell membrane , Cytoplasm, cytosol
Background
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
Database
Research Area
REFERENCE
There are currently no references for cK-Ras antibody [234-4.2] (GTX82497). Be the first to share your publications with this product.
REVIEW
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