APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
Predict Reactivity
Mouse, Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.62 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human eIF2 alpha. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950184
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
eukaryotic translation initiation factor 2 subunit alpha , EIF-2 , EIF-2A , EIF-2alpha , EIF2 , EIF2A
Background
The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-eIF2 alpha antibody [N1C1] used in Western Blot (WB). GTX112918](/templates/default/js/lightbox/images/loading.gif "Anti-eIF2 alpha antibody [N1C1] used in Western Blot (WB). GTX112918")
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GTX112918 WB Image
Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX112918 diluted at 1:2000
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GTX112918 IHC-P Image
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using eIF2 alpha(GTX112918) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for eIF2 alpha antibody [N1C1] (GTX112918). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for eIF2 alpha antibody [N1C1] (GTX112918). Be the first to share your experience with this product.
![Anti-eIF2 alpha antibody [N1C1] used in Western Blot (WB). GTX112918](/upload/website/prouct_img/small/GTX112918/GTX112918_40100_WB_w_23060500_469.webp "Anti-eIF2 alpha antibody [N1C1] used in Western Blot (WB). GTX112918")
![Anti-eIF2 alpha antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112918](/upload/website/prouct_img/small/GTX112918/GTX112918_40100_IHC_w_23060500_217.webp "Anti-eIF2 alpha antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112918")
![Anti-eIF2 alpha antibody [N1C1] used in Western Blot (WB). GTX112918](/upload/website/prouct_img/normal/GTX112918/GTX112918_40100_WB_w_23060500_469.webp "Anti-eIF2 alpha antibody [N1C1] used in Western Blot (WB). GTX112918")
![Anti-eIF2 alpha antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112918](/upload/website/prouct_img/normal/GTX112918/GTX112918_40100_IHC_w_23060500_217.webp "Anti-eIF2 alpha antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112918")