GeneTex

  • Country / Location Selection

United States (US)

eIF2 alpha antibody

Anti-eIF2 alpha antibody used in Western Blot (WB). GTX64363
Anti-eIF2 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX64363
Anti-eIF2 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX64363
Anti-eIF2 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX64363
Anti-eIF2 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64363

Cat. No. GTX64363

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all eIF2 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human EIF2S1 (NP_004085.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 subunit alpha , EIF-2 , EIF-2A , EIF-2alpha , EIF2 , EIF2A

Background

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-eIF2 alpha antibody used in Western Blot (WB). GTX64363

GTX64363 WB Image

WB analysis of various sample lysates using GTX64363 eIF2 alpha antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-eIF2 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX64363

GTX64363 IHC-P Image

IHC-P analysis of rat heart tissue using GTX64363 eIF2 alpha antibody.
Dilution : 1:100

Anti-eIF2 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX64363

GTX64363 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX64363 eIF2 alpha antibody.
Dilution : 1:100

Anti-eIF2 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX64363

GTX64363 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX64363 eIF2 alpha antibody.
Dilution : 1:100

Anti-eIF2 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64363

GTX64363 ICC/IF Image

ICC/IF analysis of PC-12 cells using GTX64363 eIF2 alpha antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

There are currently no references for eIF2 alpha antibody (GTX64363). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for eIF2 alpha antibody (GTX64363). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399