GeneTex

  • Country / Location Selection

United States (US)

eIF3H / EIF3S3 antibody [GT35512]

Anti-eIF3H / EIF3S3 antibody [GT35512] used in Western Blot (WB). GTX633631
Anti-eIF3H / EIF3S3 antibody [GT35512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633631
Anti-eIF3H / EIF3S3 antibody [GT35512] used in IHC (Paraffin sections) (IHC-P). GTX633631

Cat. No. GTX633631

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT35512

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all eIF3H / EIF3S3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human eIF3H / EIF3S3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888355

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 3 subunit H , EIF3S3 , eIF3-gamma , eIF3-p40

Cellular Localization

Cytoplasm

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Database

Research Area

DATA IMAGES

Anti-eIF3H / EIF3S3 antibody [GT35512] used in Western Blot (WB). GTX633631

GTX633631 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with eIF3H / EIF3S3 antibody [GT35512] (GTX633631) diluted at 1:1000.

Anti-eIF3H / EIF3S3 antibody [GT35512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633631

GTX633631 ICC/IF Image

eIF3H / EIF3S3 antibody [GT35512] detects eIF3H / EIF3S3 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eIF3H / EIF3S3 protein stained by eIF3H / EIF3S3 antibody [GT35512] (GTX633631) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-eIF3H / EIF3S3 antibody [GT35512] used in IHC (Paraffin sections) (IHC-P). GTX633631

GTX633631 IHC-P Image

eIF3H / EIF3S3 antibody [GT35512] detects eIF3H / EIF3S3 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
eIF3H / EIF3S3 antibody [GT35512] (GTX633631) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Jin H et al. Nat Commun 2024; 15 (1) : 2551 The EIF3H-HAX1 axis increases RAF-MEK-ERK signaling activity to promote colorectal cancer progression.
Submit a Reference

REVIEW

There are currently no reviews for eIF3H / EIF3S3 antibody [GT35512] (GTX633631). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations