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hnRNP H2 antibody

Anti-hnRNP H2 antibody used in IHC (Paraffin sections) (IHC-P). GTX106825
Anti-hnRNP H2 antibody used in Western Blot (WB). GTX106825
Anti-hnRNP H2 antibody used in Western Blot (WB). GTX106825
Anti-hnRNP H2 antibody used in IHC (Paraffin sections) (IHC-P). GTX106825
Anti-hnRNP H2 antibody used in IHC (Paraffin sections) (IHC-P). GTX106825
Anti-hnRNP H2 antibody used in IHC (Paraffin sections) (IHC-P). GTX106825

Cat. No. GTX106825

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all hnRNP H2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Mouse heart , Rat heart

Predict Reactivity

Human, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP H2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885265

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein H2 , FTP3 , HNRPH' , HNRPH2 , MRXSB , NRPH2 , hnRNPH'

Cellular Localization

Nucleus , nucleoplasm

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that binds to RNAs. It is very similar to the family member HNRPH1. This gene is thought to be involved in Fabray disease and X-linked agammaglobulinemia phenotype. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-hnRNP H2 antibody used in IHC (Paraffin sections) (IHC-P). GTX106825

GTX106825 IHC-P Image

hnRNP H2 antibody detects hnRNP H2 protein at nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
hnRNP H2 antibody (GTX106825) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-hnRNP H2 antibody used in Western Blot (WB). GTX106825

GTX106825 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP H2 antibody (GTX106825) diluted at 1:500.

Anti-hnRNP H2 antibody used in Western Blot (WB). GTX106825

GTX106825 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP H2 antibody (GTX106825) diluted at 1:500.

Anti-hnRNP H2 antibody used in IHC (Paraffin sections) (IHC-P). GTX106825

GTX106825 IHC-P Image

hnRNP H2 antibody detects hnRNP H2 protein at nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
hnRNP H2 antibody (GTX106825) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-hnRNP H2 antibody used in IHC (Paraffin sections) (IHC-P). GTX106825

GTX106825 IHC-P Image

hnRNP H2 antibody detects hnRNP H2 protein at cytoplasm and nucleus in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
hnRNP H2 antibody (GTX106825) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-hnRNP H2 antibody used in IHC (Paraffin sections) (IHC-P). GTX106825

GTX106825 IHC-P Image

hnRNP H2 antibody detects hnRNP H2 protein at nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
hnRNP H2 antibody (GTX106825) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169