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p21 Cip1 antibody [HJ21]

Anti-p21 Cip1 antibody [HJ21] used in IHC (Paraffin sections) (IHC-P). GTX34925

Cat. No. GTX34925

Host

Mouse

Clonality

Monoclonal

Clone Name

HJ21

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

Human, Mouse, Rat, Monkey, Chimpanzee
Package
100 μg ($529)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
IHC-Fr Assay dependent
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Product Note

This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. It is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant p21 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CAP20 , CDKN1 , CDKN1A , CIP1 , MDA6 , P21 , SDI1 , WAF1 , cyclin dependent kinase inhibitor 1A , p21CIP1 , p21 , p21 Cip1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-p21 Cip1 antibody [HJ21] used in IHC (Paraffin sections) (IHC-P). GTX34925

GTX34925 IHC-P Image

IHC-P analysis of bladder carcinoma tissue using GTX34925 p21 Cip1 antibody [HJ21].

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529