p21 Cip1 antibody

Name: p21 Cip1 antibody
Brand: GeneTex
SKU: GTX112898
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-p21 Cip1 antibody used in Western Blot (WB). GTX112898

Anti-p21 Cip1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112898

Anti-p21 Cip1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112898

Cat. No. GTX112898

Reference 9

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Chicken
Package	100 μl ($399), 25 μl ($169)

See all p21 Cip1 products

View product citations for antibody GTX112898 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Cat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617567

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase inhibitor 1A , CAP20 , CDKN1 , CIP1 , MDA-6 , P21 , SDI1 , WAF1 , p21CIP1

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq]

Database

Gene ID: 1026 CDKN1A
UniProt: P38936 CDKN1A

Research Area

DATA IMAGES

GTX112898 WB Image

Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody (GTX112898) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX112898 IHC-P Image

p21 Cip1 antibody detects p21 Cip1 protein at nucleus by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human normal colon tissue.
p21 Cip1 stained by p21 Cip1 antibody (GTX112898) diluted at 1:500.
Antigen Retrieval: EDTA buffer, 20 min

GTX112898 ICC/IF Image

p21 Cip1 antibody detects p21 Cip1 protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p21 Cip1 protein stained by p21 Cip1 antibody (GTX112898) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX112898 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
B: HCT116 treated with 30uM cisplatin for 24hr
12% SDS PAGE
GTX112898 diluted at 1:1000

GTX112898 IHC-P Image

p21 Cip1 antibody detects CDKN1A protein at nucleus on SkHep1xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SkHep1xenograft.
p21 Cip1 antibody (GTX112898) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min