GeneTex

  • Country / Location Selection

United States (US)

p21 Cip1 (phospho Thr145) antibody

Anti-p21 Cip1 (phospho Thr145) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32376
Anti-p21 Cip1 (phospho Thr145) antibody used in IHC (Paraffin sections) (IHC-P). GTX32376
Anti-p21 Cip1 (phospho Thr145) antibody used in Western Blot (WB). GTX32376

Cat. No. GTX32376

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all p21 Cip1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of p21 Cip1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase inhibitor 1A , CAP20 , CDKN1 , CIP1 , MDA-6 , P21 , SDI1 , WAF1 , p21CIP1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-p21 Cip1 (phospho Thr145) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32376

GTX32376 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX32376 p21 Cip1 (phospho Thr145) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-p21 Cip1 (phospho Thr145) antibody used in IHC (Paraffin sections) (IHC-P). GTX32376

GTX32376 IHC-P Image

IHC-P analysis of formalin fixed human prostate cancer tissue section using GTX32376 p21 Cip1 (phospho Thr145) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-p21 Cip1 (phospho Thr145) antibody used in Western Blot (WB). GTX32376

GTX32376 WB Image

WB analysis of EGF-treated HT1080 (A), EGF-treated HeLa (B) whole cell lysates using GTX32376 p21 Cip1 (phospho Thr145) antibody.

REFERENCE

Application Reference

Chow RY et al. Front Oncol 2021; 11 : 668247 PI3K Promotes Basal Cell Carcinoma Growth Through Kinase-Induced p21 Degradation.
Application : IHC-P
Reactivity : Human, Mouse
Submit a Reference

REVIEW

There are currently no reviews for p21 Cip1 (phospho Thr145) antibody (GTX32376). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399