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15-PGDH antibody, C-term

Anti-15-PGDH antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89634
Anti-15-PGDH antibody, C-term used in Western Blot (WB). GTX89634
Anti-15-PGDH antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89634

Cat. No. GTX89634

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all 15-PGDH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-1μg/ml
IHC-P 2-4μg/ml

Note :

IHC-P
Human Colon shows strong cytoplasm staining of enterocytes.
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89634-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DYDTTPFQAKTQ, from the C Terminus of the protein sequence according to NP_000851.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

15-hydroxyprostaglandin dehydrogenase , 15-PGDH , PGDH , PGDH1 , PHOAR1 , SDR36C1

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-15-PGDH antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89634

GTX89634 IHC-P Image

IHC-P analysis of human colon using GTX89634 15-PGDH antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-15-PGDH antibody, C-term used in Western Blot (WB). GTX89634

GTX89634 WB Image

WB analysis of human duodenum lysate using GTX89634 15-PGDH antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-15-PGDH antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89634

GTX89634 IHC-P Image

IHC-P analysis of paraffin embedded human liver using GTX89634 15-PGDH antibody, C-term.
Antigen retrieval : Steamed antigen retrieval with citrate buffer pH 6
Dilution : 2.5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399