APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 334-613 of human AIFM1 (NP_004199.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
apoptosis inducing factor mitochondria associated 1 , AIF , CMT2D , CMTX4 , COWCK , COXPD6 , DFNX5 , NADMR , NAMSD , PDCD8
Cellular Localization
Mitochondrion inner membrane,Cytoplasm,Nucleus
Background
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and cognitive disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Aug 2015]
Database
Research Area
DATA IMAGES
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GTX32428 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX32428 AIF antibody.
Blue : DAPI
Dilution : 1:200
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GTX32428 ICC/IF Image
ICC/IF analysis of C6 cells using GTX32428 AIF antibody.
Blue : DAPI
Dilution : 1:100
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GTX32428 IHC-P Image
IHC-P analysis of human thyroid cancer tissue using GTX32428 AIF antibody.
Dilution : 1:50
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GTX32428 ICC/IF Image
ICC/IF analysis of L929 cells using GTX32428 AIF antibody.
Blue : DAPI
Dilution : 1:100
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GTX32428 IHC-P Image
IHC-P analysis of mouse skeletal muscle tissue using GTX32428 AIF antibody.
Blue : DAPI
Dilution : 1:100
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GTX32428 WB Image
WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX32428 AIF antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX32428 IHC-P Image
IHC-P analysis of mouse stomach tissue using GTX32428 AIF antibody.
Dilution : 1:50
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GTX32428 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX32428 AIF antibody.
Dilution : 1:50
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GTX32428 WB Image
WB analysis of various sample lysates using GTX32428 AIF antibody.
Dilution : 1:1000
Loading : 25μg per lane
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REFERENCE
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REVIEW
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