APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88046-PEP).
Predict Reactivity
Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence CPLDALNTTKP, from the internal region of the protein sequence according to NP_006243.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein kinase AMP-activated catalytic subunit alpha 2 , AMPK , AMPK2 , AMPKa2 , PRKAA
Cellular Localization
Cytoplasm , Nucleus
Background
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX88046 WB Image
WB analysis of human liver lysate using GTX88046 AMPK alpha 2 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88046 WB Image
WB analysis of mouse (A) and rat (B) heart lysate using GTX88046 AMPK alpha 2 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88046 ELISA Image
ELISA analysis of AMPK alpha 2 protein using GTX88046 AMPK alpha 2 antibody, Internal.
Capture : GTX113251 (2.5μg/ml)
Detection : GTX88046 (1.5μg/ml)
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REFERENCE
There are currently no references for AMPK alpha 2 antibody, Internal (GTX88046). Be the first to share your publications with this product.
REVIEW
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