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APE1 antibody [2104]

Anti-APE1 antibody [2104] used in Western Blot (WB). GTX70131

Cat. No. GTX70131

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2104

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all APE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:5000
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HepG2, HeLa, Raji whole cell lysates

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human APE1 purified from E. coli

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_367806

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apurinic/apyrimidinic endodeoxyribonuclease 1 , APE , APE1 , APEN , APEX , APX , HAP1 , REF1

Cellular Localization

Nucleus , Nucleus speckle , Endoplasmic reticulum , Cytoplasm , Nucleolus

Background

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-APE1 antibody [2104] used in Western Blot (WB). GTX70131

GTX70131 WB Image

GTX70131 at a 1/1000 dilution staining ~ 38 kDa APE 1 in 35mg of:

A) HepG2 whole cell lysate; B) HeLa whole cell lysate; C) Raji whole cell lysate.

REFERENCE

Application Reference

Guo Z et al. Nucleic Acids Res 2009; 37 (10) : 3431-41 Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity.
Application : WB
Reactivity : Mouse
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Package List Price ($)
$ 399
Custom Conjugations