GeneTex

  • Country / Location Selection

United States (US)

ATR (phospho Ser428) antibody

Orthogonal
Anti-ATR (phospho Ser428) antibody used in Western Blot (WB). GTX133852
Anti-ATR (phospho Ser428) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133852
Anti-ATR (phospho Ser428) antibody used in Western Blot (WB). GTX133852
Anti-ATR (phospho Ser428) antibody used in Western Blot (WB). GTX133852

Cat. No. GTX133852

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ATR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

301 kDa. ( Note )

Positive Control

HCT116 (100 J/m2 UVC recovery 4 hr)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser428 of human ATR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887120

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATR serine/threonine kinase , FCTCS , FRP1 , MEC1 , SCKL , SCKL1

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATR (phospho Ser428) antibody used in Western Blot (WB). GTX133852

GTX133852 WB Image

Orthogonal

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Ser428) antibody (GTX133852) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATR (phospho Ser428) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133852

GTX133852 ICC/IF Image

ATR (phospho Ser428) antibody detects ATR (phospho Ser428) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATR (phospho Ser428) stained by ATR (phospho Ser428) antibody (GTX133852) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Scale bar= 10 μm.

Anti-ATR (phospho Ser428) antibody used in Western Blot (WB). GTX133852

GTX133852 WB Image

Orthogonal

Untreated (–) and treated (+) mouse tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Ser428) antibody (GTX133852) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ATR (phospho Ser428) antibody used in Western Blot (WB). GTX133852

GTX133852 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Ser428) antibody (GTX133852) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for ATR (phospho Ser428) antibody (GTX133852). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for ATR (phospho Ser428) antibody (GTX133852). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169