APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
HCT116 , HCT116(100 J/m2 UVC and recover for 6hr)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Thr1989 of human ATR. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888260
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATR serine/threonine kinase , FCTCS , FRP1 , MEC1 , SCKL , SCKL1
Cellular Localization
Nucleus
Background
The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX631845 IP Image
Immunoprecipitation of ATR protein from HCT-116 whole cell extracts treated with 2 mM Hydroxyurea 6 hrs using 5 μg of ATR antibody (GTX631845) or ATR antibody (GTX128146). Western blot analysis was performed using ATR antibody (GTX631845) diluted at 1:500. EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
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GTX631845 WB Image
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [GT222] (GTX631845) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
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GTX631845 ICC/IF Image
ATR (phospho Thr1989) antibody [GT222] detects ATR (phospho Thr1989) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [GT222] (GTX631845) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (GTX102079) diluted at 1:1000.
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GTX631845 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [GT222] (GTX631845) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX631845 ICC/IF Image
ATR (phospho Thr1989) antibody [GT222] detects ATR (phospho Thr1989) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [GT222] (GTX631845) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
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REVIEW
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