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Adenosine A2b Receptor antibody, C-term

Anti-Adenosine A2b Receptor antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89506
Anti-Adenosine A2b Receptor antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89506
Anti-Adenosine A2b Receptor antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89506
Anti-Adenosine A2b Receptor antibody, C-term used in Flow cytometry (FACS). GTX89506

Cat. No. GTX89506

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($399)
See all Adenosine A2b Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 10μg/ml
IHC-P 2-4μg/ml
FACS Assay dependent

Note :

ICC/IF
Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells.

IHC-P
Human Kidney shows cytoplasm staining in cells of renal tubules.
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89506-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence CQADVKSGNGQ, from the C Terminus of the protein sequence according to NP_000667.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosine A2b receptor , ADORA2

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 136 ADORA2B
  • UniProt: P29275 ADORA2B

Research Area

DATA IMAGES

Anti-Adenosine A2b Receptor antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89506

GTX89506 ICC/IF Image

ICC/IF analysis of PFA fixed U2OS cells using GTX89506 Adenosine A2b Receptor antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-Adenosine A2b Receptor antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89506

GTX89506 IHC-P Image

IHC-P analysis of human kidney using GTX89506 Adenosine A2b Receptor antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml

Anti-Adenosine A2b Receptor antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89506

GTX89506 ICC/IF Image

ICC/IF analysis of PFA fixed A431 cells using GTX89506 Adenosine A2b Receptor antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-Adenosine A2b Receptor antibody, C-term used in Flow cytometry (FACS). GTX89506

GTX89506 FACS Image

FACS analysis of PFA fixed Kelly cells using GTX89506 Adenosine A2b Receptor antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399