Adenosine A2b Receptor antibody

Cat. No. GTX04603

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Mouse, Rat
Package	50 μl ($669)

See all Adenosine A2b Receptor products

Datasheet

View product citations for antibody GTX04603 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	Assay dependent
FACS	Assay dependent

Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide KDSATNN*STEPWDGTTNESC, corresponding to amino acid residues 147-166 of human A2BAR with replacement of cysteine 154 (C154) with serine (*S) (Accession P29275).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosine A2b receptor , ADORA2

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Database

Gene ID: 136 ADORA2B
UniProt: P29275 ADORA2B

Research Area

DATA IMAGES

GTX04603 IHC-P Image

IHC-P analysis of rat lung tissue using GTX04603 Adenosine A2b Receptor antibody. Staining is present in the respiratory epithelium of the bronchiole (Br) as well as in the pneumonocytes of the alveolar wall (alveoli, (A).
Dilution : 1:50

GTX04603 FACS Image

FACS analysis of the live intact THP-1 cells using GTX04603 Adenosine A2b Receptor antibody.
Green : Primary antibody
Pink : Secondary antibody only
Black : Unlabeled cells
Dilution : 2.5 μg

GTX04603 WB Image

WB analysis of various samples using GTX04603 Adenosine A2b Receptor antibody prior incubated with (Lane 2, 4, 6) or without (Lane 1, 3, 5) blocking peptide.
Lane 1 and 2 : HL-60 whole cell lysate
Lane 3 and 4 : Rat brain tissue lysate
Lane 5 and 6 : Mouse brain tissue lysate
Dilution : 1:200

GTX04603 ICC/IF Image

ICC/IF analysis of PC12 cells using GTX04603 Adenosine A2b Receptor antibody.
Panel A : Extracellular staining of cells
Panel B : Merge of A and live view of the cells
Dilution : 1:100

REFERENCE

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Package

List Price ($)

50 μl

$ 669

Qty