GeneTex

  • Country / Location Selection

United States (US)

Annexin A1 antibody [3A8]

KO/KD Overexpression
Anti-Annexin A1 antibody [3A8] used in Western Blot (WB). GTX84886
Anti-Annexin A1 antibody [3A8] used in IHC (Paraffin sections) (IHC-P). GTX84886
Anti-Annexin A1 antibody [3A8] used in Western Blot (WB). GTX84886
Anti-Annexin A1 antibody [3A8] used in IHC (Paraffin sections) (IHC-P). GTX84886
Anti-Annexin A1 antibody [3A8] used in IHC (Paraffin sections) (IHC-P). GTX84886
Anti-Annexin A1 antibody [3A8] used in Western Blot (WB). GTX84886
Anti-Annexin A1 antibody [3A8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84886

Cat. No. GTX84886

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3A8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat, Monkey
Package
100 μl ($519)
See all Annexin A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human ANXA1 (NP_000691) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

annexin A1 , ANX1 , LPC1

Cellular Localization

Nucleus,Cytoplasm,Cell membrane,Membrane,Endosome membrane,Apical cell membrane,Secreted

Background

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

Database

Research Area

DATA IMAGES

Anti-Annexin A1 antibody [3A8] used in Western Blot (WB). GTX84886

GTX84886 WB Image

KO/KD

WB analysis of wild type and Annexin A1 knock out HeLa cell lysate(10μg per lane) using GTX84886 Annexin A1 antibody [3A8].
Dilution : 1:500

Anti-Annexin A1 antibody [3A8] used in IHC (Paraffin sections) (IHC-P). GTX84886

GTX84886 IHC-P Image

IHC-P analysis of breast tissue using GTX84886 Annexin A1 antibody [3A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Annexin A1 antibody [3A8] used in Western Blot (WB). GTX84886

GTX84886 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Annexin A1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84886 Annexin A1 antibody [3A8].
Loading : 5 ug per lane

Anti-Annexin A1 antibody [3A8] used in IHC (Paraffin sections) (IHC-P). GTX84886

GTX84886 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84886 Annexin A1 antibody [3A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Annexin A1 antibody [3A8] used in IHC (Paraffin sections) (IHC-P). GTX84886

GTX84886 IHC-P Image

IHC-P analysis of kidney tissue using GTX84886 Annexin A1 antibody [3A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Annexin A1 antibody [3A8] used in Western Blot (WB). GTX84886

GTX84886 WB Image

WB analysis of various cell lines using GTX84886 Annexin A1 antibody [3A8].
Loading : 35 ug per lane
Dilution : 1:200

Anti-Annexin A1 antibody [3A8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84886

GTX84886 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Annexin A1 plasmid using GTX84886 Annexin A1 antibody [3A8].

REFERENCE

There are currently no references for Annexin A1 antibody [3A8] (GTX84886). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Annexin A1 antibody [3A8] (GTX84886). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519