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BRN3A antibody

Anti-BRN3A antibody used in Western Blot (WB). GTX04795
Anti-BRN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX04795
Anti-BRN3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04795
Anti-BRN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX04795

Cat. No. GTX04795

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Predict Reactivity

Rabbit, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human POU4F1(Accession Q01851), corresponding to amino acid residues G164-L214.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRN3A,OctT1,POU class 4 homeobox 1,POU4F1,RDC1,brn3A

Cellular Localization

Nucleus

Background

This gene encodes a member of the POU-IV class of neural transcription factors. This protein is expressed in a subset of retinal ganglion cells and may be involved in the developing sensory nervous system. This protein may also promote the growth of cervical tumors. A translocation of this gene is associated with some adult acute myeloid leukemias. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-BRN3A antibody used in Western Blot (WB). GTX04795

GTX04795 WB Image

WB analysis of mouse brain tissue lysate using GTX04795 BRN3A antibody. The lane on the right was treated with blocking peptide.

Anti-BRN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX04795

GTX04795 IHC-P Image

IHC-P analysis of rat brain tissue using GTX04795 BRN3A antibody.
Antigen retieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-BRN3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04795

GTX04795 ICC/IF Image

ICC/IF analysis of PFA-fixed A549 cells using GTX04795 BRN3A antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

Anti-BRN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX04795

GTX04795 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX04795 BRN3A antibody.
Antigen retieval : Heat mediated citrate buffer
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399