APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 - 2 μg/mL |
20 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Brain Tissue Slide(GTX22203) , 3T3 (NIH) Cell Lysate(GTX27179)
Product Note
At least two isoforms are known to exist. This antibody will recognize both isoforms. This antibody is predicted to not cross-react with other members of the POU domain class 3 family.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Rabbit polyclonal Brn2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human Brn2.The immunogen is located within the last 50 amino acids of Brn2.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
POU class 3 homeobox 2 , BRN2 , N-Oct3 , OCT7 , OTF-7 , OTF7 , POUF3 , brn-2 , oct-7
Cellular Localization
Nucleus
Background
This gene encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an increase in the proliferation of melanoma cells. [provided by RefSeq, Mar 2012]
Database
Research Area
DATA IMAGES
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GTX31506 WB Image
WB analysis of 3T3 cell lysate using GTX31506 Brn2 antibody. Working concentration : (A) 1 and (B) 2 μg/ml
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GTX31506 IHC-P Image
IHC-P analysis of human brain tissue using GTX31506 Brn2 antibody. Working concentration : 20 μg/ml
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REFERENCE
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REVIEW
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