CARM1 antibody [3H2]

Cat. No. GTX80395

Host	Mouse
Clonality	Monoclonal
Clone Name	3H2
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse, Rat, Monkey
Package	100 μl ($399)

See all CARM1 products

Datasheet
Document

View product citations for antibody GTX80395 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CARM1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coactivator associated arginine methyltransferase 1 , PRMT4

Cellular Localization

Nucleus,Cytoplasm

Background

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).[supplied by OMIM, Mar 2008]

Database

Gene ID: 10498 CARM1
UniProt: Q86X55 CARM1

Research Area

DATA IMAGES

GTX80395 FACS Image

FACS analysis of Lovo cells using GTX80395 CARM1 antibody [3H2].
Green : CARM1
Purple : negative control

GTX80395 WB Image

WB analysis of MCF-7 (1), HeLa (2), NIH3T3 (3), HL-60 (4), LNcap (5), Jurkat (6), PC-3 (7), Cos7 (8), and PC-12 (9) cell lysate using GTX80395 CARM1 antibody [3H2].

GTX80395 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX80395 CARM1 antibody [3H2].
Green : CARM1
Red: Actin filaments

GTX80395 IHC-P Image

IHC-P analysis of breast cancer tissue (left) and ovarian cancer tissue (right) using GTX80395 CARM1 antibody [3H2].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty