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CD11b antibody [HL3097]

Recombinant antibody Orthogonal
Anti-CD11b antibody [HL3097] used in Western Blot (WB). GTX640543

Cat. No. GTX640543

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL3097

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all CD11b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Product Note

This antibody was raised against mouse CD11b Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of mouse CD11b. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin alpha M , CD11b/CD18 , CR3 , CR3A , Cd11b , F730045J24Rik , Ly-40 , MAC1 , Mac-1 , Mac-1a

Cellular Localization

Cell membrane

Background

Enables complement component C3b binding activity; heparan sulfate proteoglycan binding activity; and heparin binding activity. Contributes to cargo receptor activity. Involved in several processes, including central nervous system development; endocytosis; and positive regulation of neuron death. Acts upstream of or within several processes, including activated T cell proliferation; leukocyte migration; and microglia development. Located in external side of plasma membrane and nucleus. Is integral component of membrane. Part of integrin alphaM-beta2 complex. Is expressed in several structures, including central nervous system; heart; lymphatic vessel; thymus; and yolk sac. Human ortholog(s) of this gene implicated in lupus nephritis. Orthologous to human ITGAM (integrin subunit alpha M). [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-CD11b antibody [HL3097] used in Western Blot (WB). GTX640543

GTX640543 WB Image

Orthogonal

Boiled and unboiled Raw264.7 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD11b antibody [HL3097] (GTX640543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Available soon.
Custom Conjugations