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CD28 antibody [CD28.2] (Biotin)

Anti-CD28 antibody [CD28.2] (Biotin) used in Flow cytometry (FACS). GTX03365

Cat. No. GTX03365

Reference 14

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 CD28.2

Isotype

 IgG1

Application

 FACS

Reactivity

 Human, Baboon, Chimpanzee, Cynomolgus monkey, Rhesus Monkey
Package
100 μg ($339)
See all CD28 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human CD28 transfected murine T cell hybridoma.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Biotin

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD28 molecule , Tp44

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES

Anti-CD28 antibody [CD28.2] (Biotin) used in Flow cytometry (FACS). GTX03365

GTX03365 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX03365 CD28 antibody [CD28.2] (Biotin).
Solid line : Primary antibody
Dashed line : Isotype control
Antibody amount : 0.25μg

REFERENCE

Clone Reference

2020; TRIM34 restricts HIV-1 and SIV capsids in a TRIM5α-dependent manner
2019; A predicted Francisella tularensis DXD-motif glycosyltransferase blocks immune activation
2019; Contribution of ROS and metabolic status to neonatal and adult CD8+ T cell activation
2014; The Effects of Age and Cytomegalovirus on Markers of Inflammation and Lymphocyte Populations in Captive Baboons
2013; Protection against H5N1 highly pathogenic avian and pandemic (H1N1) 2009 influenza virus infection in cynomolgus monkeys by an inactivated H5N1 whole particle vaccine
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REVIEW

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Clone Reference
2020; TRIM34 restricts HIV-1 and SIV capsids in a TRIM5α-dependent manner
2019; A predicted Francisella tularensis DXD-motif glycosyltransferase blocks immune activation
2019; Contribution of ROS and metabolic status to neonatal and adult CD8+ T cell activation
2014; The Effects of Age and Cytomegalovirus on Markers of Inflammation and Lymphocyte Populations in Captive Baboons
2013; Protection against H5N1 highly pathogenic avian and pandemic (H1N1) 2009 influenza virus infection in cynomolgus monkeys by an inactivated H5N1 whole particle vaccine
2012; Soluble factors from T cells inhibiting X4 strains of HIV are a mixture of β chemokines and Rnases
2012; Naive T cells are dispensable for memory CD4+ T cell homeostasis in progressive simian immunodeficiency virus infection
2012; Treatment of SIV-infected sooty mangabeys with a type-I IFN agonist results in decreased virus replication without inducing hyperimmune activation
2011; Blocking of α4β7 gut-homing integrin during acute infection leads to decreased plasma and gastrointestinal tissue viral loads in simian immunodeficiency virus-infected rhesus macaques
2010; Relative over-reactivity of human versus chimpanzee lymphocytes: implications for the human diseases associated with immune activation
2009; The lack of epithelial interleukin-7 and BAFF/BLyS gene expression in prostate cancer as a possible mechanism of tumor escape from immunosurveillance
2008; Regulation of CD28 expression on CD8+ T cells by CTLA-4
2008; ICOS ligation recruits the p50alpha PI3K regulatory subunit to the immunological synapse
2000; Interelationship between CD3 and CD28 pathways in a murine T cell thymoma
Package List Price ($)
$ 339