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CD31 antibody [TLD-3A12] (Low endotoxin, azide free)

Anti-CD31 antibody [TLD-3A12] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42089
Anti-CD31 antibody [TLD-3A12] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42089

Cat. No. GTX42089

Reference 12

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 TLD-3A12

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA

Reactivity

 Rat, Pig, Rhesus Monkey
Package
500 μg ($759)
See all CD31 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 1/50-1/100
ELISA Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Activated, Lewis rat derived microglial cells.

Purification

Protein G purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

RRID

AB_11174106

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

platelet and endothelial cell adhesion molecule 1 , CD31 , Pecam

Cellular Localization

Cell membrane , Cell junction

Background

cell adhesion molecule, also intracellular signaling molecule; may mediate leukocyte adhesion and migration, angiogenesis, and thrombosis [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-CD31 antibody [TLD-3A12] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42089

GTX42089 IHC-Fr Image

IHC-Fr analysis of rat spleen tissue using GTX42089 CD31 antibody [TLD-3A12] (Low endotoxin, azide free).

Anti-CD31 antibody [TLD-3A12] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42089

GTX42089 IHC-Fr Image

IHC-Fr analysis of rat brain tissue using GTX42089 CD31 antibody [TLD-3A12] (Low endotoxin, azide free).

REFERENCE

Application Reference

Bai Y et al. Front Physiol 2022; 13 : 913399 Efficacy of Shexiang Tongxin Dropping Pills in a Swine Model of Coronary Slow Flow.
Application : WB
Reactivity : Pig
Mahara A et al. Biomacromolecules 2020; 21 (8) : 3092-3101 Small-Diameter Synthetic Vascular Graft Immobilized with the REDV Peptide Reduces Early-Stage Fibrin Clot Deposition and Results in Graft Patency in Rats.
Application : IHC-Fr
Reactivity : Rat

Clone Reference

Kant V et al. Immunopharmacol Immunotoxicol 2021; 43 (5):536-553 Topical application of quercetin improves wound repair and regeneration in diabetic rats.
Kant V et al. Growth Factors 2020; 38 (2):105-119 Quercetin accelerated cutaneous wound healing in rats by modulation of different cytokines and growth factors.
Nakakura T et al. Cell Tissue Res 2020; (Epub) Fibronectin is essential for formation of fenestrae in endothelial cells of the fenestrated capillary.
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REVIEW

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Application Reference
Bai Y et al. Front Physiol 2022; 13:913399 Efficacy of Shexiang Tongxin Dropping Pills in a Swine Model of Coronary Slow Flow.
Application : WB
Reactivity : Pig
Mahara A et al. Biomacromolecules 2020; 21 (8):3092-3101 Small-Diameter Synthetic Vascular Graft Immobilized with the REDV Peptide Reduces Early-Stage Fibrin Clot Deposition and Results in Graft Patency in Rats.
Application : IHC-Fr
Reactivity : Rat
Ho YJ et al. Biomaterials 2020; 232:119723 Superhydrophobic drug-loaded mesoporous silica nanoparticles capped with β-cyclodextrin for ultrasound image-guided combined antivascular and chemo-sonodynamic therapy.
Matsuzaki K et al. FEBS openbio 2017; (Epub) Daily voluntary exercise enhances pilocarpine-induced saliva secretion and aquaporin 1 expression in rat submandibular glands.
Application : WB
Reactivity : Rat
Chen CK et al. Mol Neurobiol 2017; (Epub) TRPV4 Activation Contributes Functional Recovery from Ischemic Stroke via Angiogenesis and Neurogenesis.
Application : IHC-Fr
Reactivity : Rat
Vitacolonna M et al. Cell Tissue Bank 2016; (Epub) In-vivo quantification of the revascularization of a human acellular dermis seeded with EPCs and MSCs in co-culture with fibroblasts and pericytes in the dorsal chamber model in pre-irradiated tissue.
Application : ICC/IF
Reactivity : Rat
Xi Y et al. Sci Rep 2016; 6:32424 FSTL1 as a Potential Mediator of Exercise-Induced Cardioprotection in Post-Myocardial Infarction Rats.
Application : IHC-P
Reactivity : Rat
Chen CJ et al. J Virol 2014; 88 (2):1150-61 Infection of pericytes in vitro by Japanese encephalitis virus disrupts the integrity of the endothelial barrier.
Application : FACS
Reactivity : Rat
Agudelo CA et al. J Biomater Appl 2013; 28 (3):473-80 Synthesis, properties, and endothelial progenitor cells labeling stability of dextranes as polymeric magnetic resonance imaging contrast agents.
Application : FACS
Reactivity : Rat
Clone Reference
Kant V et al. Immunopharmacol Immunotoxicol 2021; 43 (5):536-553 Topical application of quercetin improves wound repair and regeneration in diabetic rats.
Kant V et al. Growth Factors 2020; 38 (2):105-119 Quercetin accelerated cutaneous wound healing in rats by modulation of different cytokines and growth factors.
Nakakura T et al. Cell Tissue Res 2020; (Epub) Fibronectin is essential for formation of fenestrae in endothelial cells of the fenestrated capillary.
SDS
PBS.pdf
Package List Price ($)
$ 759
Custom Conjugations