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CD45 antibody [IBL-3/16]

Cat. No. GTX74961

Reference 2

Host

 Rat

Clonality

 Monoclonal

Clone Name

 IBL-3/16

Isotype

 IgG1

Application

 ICC/IF, IHC-Fr, FACS, IP, IHC

Reactivity

 Mouse
Package
250 μg ($459)
See all CD45 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-Fr 1/50-1/100
FACS 1/50-1/100
IP Assay dependent
IHC Assay dependent

Note :

IHC-Fr
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

145 kDa. ( Note )

Product Note

This antibody is expected to regognize all isoforms of murine CD45.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Purified B cells from mouse lymph nodes.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_378308

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase, receptor type, C , B220 , CD45R , Cd45 , L-CA , Ly-5 , Lyt-4 , T200 , loc

Cellular Localization

Cell membrane

Database

Research Area

REFERENCE

Application Reference

Gomi M et al. J Neurosci Res 2011; 89 (9) : 1388-99 Single and local blockade of interleukin-6 signaling promotes neuronal differentiation from transplanted embryonic stem cell-derived neural precursor cells.
Application : IHC
Uemura M et al. J Neurosci Res 2010; 88 (3) : 542-51 Matrigel supports survival and neuronal differentiation of grafted embryonic stem cell-derived neural precursor cells.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459