APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Neat-1/10 |
Note :
FACS
Use 10μl of the suggested working dilution to label 100μl whole blood.
|
Not tested in other applications.
Product Note
This antibody reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes.
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.
Concentration
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human T lymphocytes
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Fluorescein isothiocyanate (FITC)
RRID
AB_366756
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD45 Antibody , LY5 Antibody , T200 Antibody , L-CA Antibody , PTPRC Antibody , LCA Antibody , CD45R Antibody , protein tyrosine phosphatase, receptor type, C Antibody , B220 Antibody , GP180 Antibody
Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Research Area
DATA IMAGES
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GTX76597 FACS Image
Figure A. PE-Cy5 conjugated mouse anti human CD45RO (GTX76156) and FITC conjugated Mouse IgG1 isotype control (GTX76638). Figure B. PE-Cy5 conjugated mouse anti human CD45RO (GTX76156) and FITC conjugated mouse anti human CD45RA (GTX76597). All experiments performed on red cell lysed human peripheral blood gated on lymphocytes.
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REFERENCE
There are currently no references for CD45RA antibody [F8-11-13] (FITC) (GTX76597). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD45RA antibody [F8-11-13] (FITC) (GTX76597). Be the first to share your experience with this product.