APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Neat-1/5 |
|
Note :
FACS
Membrane permeabilisation may enhance CD74 staining. Use 10μl of the suggested working dilution to label 106 cells in 100μl.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes 33, 35 and 41 kD isoforms of CD74. Clone Bu45 binds to an epitope within the extracytoplasmic domain of the CD74 molecule, which is distal from the C-terminus.
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.
Concentration
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
B lymphoblastoid cell line HFB1.
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Fluorescein isothiocyanate (FITC)
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD74 molecule , DHLAG , HLADG , II , Ia-GAMMA , p33
Cellular Localization
Cell membrane , Endoplasmic reticulum membrane , Endosome , Lysosome
Background
The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
Database
Research Area
DATA IMAGES
![Anti-CD74 antibody [Bu45] (FITC) used in Flow cytometry (FACS). GTX82972](/templates/default/js/lightbox/images/loading.gif "Anti-CD74 antibody [Bu45] (FITC) used in Flow cytometry (FACS). GTX82972")
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GTX82972 FACS Image
Figure A. RPE-A750 tandem conjugated Mouse anti Human CD19 and FITC conjugated Mouse IgG1 isotype control (GTX76638). Figure B. RPE-A750 tandem conjugated Mouse anti Human CD19 and FITC conjugated Mouse anti Human CD74 (GTX82972). All experiments performed on human Peripheral blood lymphocytes.
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REFERENCE
There are currently no references for CD74 antibody [Bu45] (FITC) (GTX82972). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD74 antibody [Bu45] (FITC) (GTX82972). Be the first to share your experience with this product.
![Anti-CD74 antibody [Bu45] (FITC) used in Flow cytometry (FACS). GTX82972](/upload/website/prouct_img/small/GTX82972/GTX82972_2761_FACS_w_23061322_326.webp "Anti-CD74 antibody [Bu45] (FITC) used in Flow cytometry (FACS). GTX82972")
![Anti-CD74 antibody [Bu45] (FITC) used in Flow cytometry (FACS). GTX82972](/upload/website/prouct_img/normal/GTX82972/GTX82972_2761_FACS_w_23061322_326.webp "Anti-CD74 antibody [Bu45] (FITC) used in Flow cytometry (FACS). GTX82972")