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CD86 antibody [C86/1146]

Anti-CD86 antibody [C86/1146] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34569
Anti-CD86 antibody [C86/1146] used in Western Blot (WB). GTX34569
Anti-CD86 antibody [C86/1146] used in IHC (Paraffin sections) (IHC-P). GTX34569

Cat. No. GTX34569

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 C86/1146

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μg ($529)
See all CD86 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 2-4μg/ml for 30 minutes at RT

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human full-length CD86 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

B7.2 , B70 , B72 , CD28LG2 , CD86 , CD86 molecule , LAB72 , B7-2

Cellular Localization

Cell membrane

Background

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-CD86 antibody [C86/1146] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34569

GTX34569 ICC/IF Image

ICC/IF analysis of Raji cells using GTX34569 CD86 antibody [C86/1146].
Green : Primary antibody
Red : nucleus

Anti-CD86 antibody [C86/1146] used in Western Blot (WB). GTX34569

GTX34569 WB Image

WB analysis of Daudi cell lysate using GTX34569 CD86 antibody [C86/1146].

Anti-CD86 antibody [C86/1146] used in IHC (Paraffin sections) (IHC-P). GTX34569

GTX34569 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX34569 CD86 antibody [C86/1146].

REFERENCE

Application Reference

Ko CY et al. Int J Biol Sci 2023; 19 (16) : 5275-5289 Omentin-1 ameliorates the progress of osteoarthritis by promoting IL-4-dependent anti-inflammatory responses and M2 macrophage polarization.
Yunke Liu et al. J Healthc Eng 2022; 2022 : 4441603 Impact of Yiqi Huoxue Decoction on the Relationship between Remodeling of Cardiac Nerves and Macrophages after Myocardial Infarction in Rats.
Application : WB
Fang SY et al. Exp Ther Med 2021; 21 (4) : 369 Distinct phenotypic expression levels of macrophages in neonatal lungs.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529