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CD97 antibody [C3], C-term

Anti-CD97 antibody [C3], C-term used in Western Blot (WB). GTX108192
Anti-CD97 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108192
Anti-CD97 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108192

Cat. No. GTX108192

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CD97 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

HepG2 , HCT116

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human CD97. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036526

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adhesion G protein-coupled receptor E5 , CD97 , TM7LN1

Cellular Localization

Cell membrane , CD97 antigen subunit alpha: Secreted , extracellular space

Background

This gene is a member of the EGF-TM7 family of class II seven-span transmembrane (7-TM) molecules, likely encoded by a gene cluster on the short arm of chromosome 19. The encoded product is a glycoprotein that is present on the surface of most activated leukocytes and spans the membrane seven times, which is a defining feature of G protein-coupled receptors. The protein has an extended extracellular region with several N-terminal epidermal growth factor (EGF)-like domains, which mediate binding to its cellular ligand, decay accelerating factor (DAF, CD55), a regulatory protein of the complement cascade. The presence of structural features characteristic of extracellular matrix proteins and transmembrane proteins suggests that this protein is a receptor involved in both cell adhesion and signaling processes early after leukocyte activation. Alternative splicing has been observed for this gene and three variants have been found. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD97 antibody [C3], C-term used in Western Blot (WB). GTX108192

GTX108192 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: HCT116
7.5% SDS PAGE
GTX108192 diluted at 1:1000

Anti-CD97 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108192

GTX108192 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV21G xenograft, using CD97(GTX108192) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD97 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108192

GTX108192 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using CD97(GTX108192) antibody at 1:200 dilution.

REFERENCE

Application Reference

Su Q et al. World J Surg Oncol 2022; 20 (1) : 382 CD97 serves as a novel biomarker of immune cell infiltration in hepatocellular carcinoma.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169