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CLGN antibody [9C8G10]

Anti-CLGN antibody [9C8G10] used in ELISA (ELISA). GTX60730
Anti-CLGN antibody [9C8G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60730
Anti-CLGN antibody [9C8G10] used in IHC (Paraffin sections) (IHC-P). GTX60730
Anti-CLGN antibody [9C8G10] used in Western Blot (WB). GTX60730
Anti-CLGN antibody [9C8G10] used in IHC (Paraffin sections) (IHC-P). GTX60730
Anti-CLGN antibody [9C8G10] used in Flow cytometry (FACS). GTX60730
Anti-CLGN antibody [9C8G10] used in Western Blot (WB). GTX60730

Cat. No. GTX60730

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9C8G10

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($409)
See all CLGN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/150
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CLGN (AA: 249-405) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calmegin

Background

Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CLGN antibody [9C8G10] used in ELISA (ELISA). GTX60730

GTX60730 ELISA Image

ELISA analysis of antigen using GTX60730 CLGN antibody [9C8G10].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-CLGN antibody [9C8G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60730

GTX60730 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60730 CLGN antibody [9C8G10].
Green : CLGN
Blue: DRAQ5 fluorescent DNA dye

Anti-CLGN antibody [9C8G10] used in IHC (Paraffin sections) (IHC-P). GTX60730

GTX60730 IHC-P Image

IHC-P analysis of esophageal cancer tissue using GTX60730 CLGN antibody [9C8G10].

Anti-CLGN antibody [9C8G10] used in Western Blot (WB). GTX60730

GTX60730 WB Image

WB analysis of HEK293 (1) and CLGN (AA: 249-405)-hIgGFc transfected HEK293 (2) cell lysate using GTX60730 CLGN antibody [9C8G10].

Anti-CLGN antibody [9C8G10] used in IHC (Paraffin sections) (IHC-P). GTX60730

GTX60730 IHC-P Image

IHC-P analysis of breast cancer tissue using GTX60730 CLGN antibody [9C8G10].

Anti-CLGN antibody [9C8G10] used in Flow cytometry (FACS). GTX60730

GTX60730 FACS Image

FACS analysis of HEK293 cells using GTX60730 CLGN antibody [9C8G10].
Green : CLGN
Purple : negative control

Anti-CLGN antibody [9C8G10] used in Western Blot (WB). GTX60730

GTX60730 WB Image

WB analysis of LNCaP (1), HepG2 (2), PC-3 (3), and Raji (4) cell lysate using GTX60730 CLGN antibody [9C8G10].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409