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COPA antibody

Anti-COPA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22913

Cat. No. GTX22913

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Rat, Primate
Package
100 μg ($399)
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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ICC/IF 3 μg/ml
IP Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide corresponding to residues M(1) L T K F E T K S A R V K G L S F H P(19) C of rat alpha-COP.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_384906

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coatomer protein complex subunit alpha

Background

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits.

Database

Research Area

DATA IMAGES

Anti-COPA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22913

GTX22913 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22913 COPA antibody.

REFERENCE

Application Reference

Austin CD et al. Proc Natl Acad Sci U S A 2006; 103 (27) : 10283-8 Death-receptor activation halts clathrin-dependent endocytosis.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399