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CRYZ antibody [N1C1]

Anti-CRYZ antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX118270
Anti-CRYZ antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118270
Anti-CRYZ antibody [N1C1] used in Western Blot (WB). GTX118270
Anti-CRYZ antibody [N1C1] used in Western Blot (WB). GTX118270

Cat. No. GTX118270

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CRYZ products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

Jurkat , Raji , Mouse brain

Predict Reactivity

Rat, Bovine, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CRYZ. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722810

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

crystallin zeta

Cellular Localization

Cytoplasm

Background

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. The former class is also called phylogenetically-restricted crystallins. This gene encodes a taxon-specific crystallin protein which has NADPH-dependent quinone reductase activity distinct from other known quinone reductases. It lacks alcohol dehydrogenase activity although by similarity it is considered a member of the zinc-containing alcohol dehydrogenase family. Unlike other mammalian species, in humans, lens expression is low. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One pseudogene is known to exist. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CRYZ antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX118270

GTX118270 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using zeta Crystallin(GTX118270) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CRYZ antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118270

GTX118270 ICC/IF Image

zeta Crystallin antibody [N1C1] detects zeta Crystallin protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: zeta Crystallin protein stained by zeta Crystallin antibody [N1C1] (GTX118270) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-CRYZ antibody [N1C1] used in Western Blot (WB). GTX118270

GTX118270 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX118270 diluted at 1:500

Anti-CRYZ antibody [N1C1] used in Western Blot (WB). GTX118270

GTX118270 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
10% SDS PAGE
GTX118270 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169