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Calpain 9 antibody [N1N3]

Anti-Calpain 9 antibody [N1N3] used in Western Blot (WB). GTX115472
Anti-Calpain 9 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115472

Cat. No. GTX115472

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Calpain 9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Calpain 9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731587

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain 9 , GC36 , nCL-4

Background

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calpain 9 antibody [N1N3] used in Western Blot (WB). GTX115472

GTX115472 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX115472 diluted at 1:3000

Anti-Calpain 9 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115472

GTX115472 ICC/IF Image

Calpain 9 antibody [N1N3] detects Calpain 9 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calpain 9 protein stained by Calpain 9 antibody [N1N3] (GTX115472) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169